Review




Structured Review

Croda International Plc hand extruder
Hand Extruder, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 96/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hand+extruder/PC+Membranes/pm42031174-338-6-8
Average 96 stars, based on 221 article reviews
hand extruder - by Bioz Stars, 2026-09
96/100 stars

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Related Articles

Liposomes:

Article Title: Biochemical Functions of the Membrane-Binding Domain of CARMIL
Article Snippet: .. Liposomes were then passed through a hand-extruder (Genizer, Avanti Research, #610000, Alabaster, AL) and through a 0.2 μm polycarbonate membrane (Avanti Research, #610006, Alabaster, AL) nine times and collected for use. ..

Article Title: Structural and biochemical analysis of ligand binding in yeast Niemann-Pick type C1-related protein
Article Snippet: .. Before use, liposomes were extruded by passing 20x through a 100 nm pore size polycarbonate filter (Whatman, Nucleopore) using a hand extruder (Avanti Polar Lipids). ..

Article Title: CARMIL Membrane-Binding Domain Regulates Capping Protein and Actin Assembly.
Article Snippet: .. Liposomes were then passed through a hand-extruder (Genizer, Avanti Research, #610000, Alabaster, AL) and through a 0.2 μm polycarbonate membrane (Avanti Research, #610006, Alabaster, AL) nine times and collected for use. ..

Article Title: Yck3 casein kinase-mediated phosphorylation determines Ivy1 localization and function at endosomes and the vacuole.
Article Snippet: The Saccharomyces cerevisiae casein kinase protein Yck3 is a central regulator at the vacuole that phosphorylates several proteins involved in membrane trafficking.. Here, we set out to identify novel substrates of this protein.. We found that endogenously tagged Yck3 localized not only at the vacuole, but also on endosomes.

Article Title: The sterol transporter STARD3 transports sphingosine at ER-lysosome contact sites
Article Snippet: .. Before use, liposomes were extruded by passing 20x through a 100 nm pore size polycarbonate filter (Whatman - Nuclepore) using a hand extruder (Avanti Polar Lipids). ..

Article Title: VAP-A intrinsically disordered regions enable versatile tethering at membrane contact sites.
Article Snippet: .. Liposomes were extruded through 0.1 mm pore size polycarbonate filters using hand extruder (Avanti Polar Lipids) and were used within 1-2 days. .. Full length VAP-A reconstitution into proteoliposomes VAP-AFL WT or sL1/2 were reconstituted by detergent removal using BioBeads as described previously in lipid mixture containing egg phosphatidylcholine (PC) and brain phosphatidylserine (PS) (95/5 mol%) for cryo-EM experiment or egg PC, brain PS, DGS NTA(Ni), cholesterol (93/5/2/10 mol%) for in vitro lipid transfer assay.

Membrane:

Article Title: Biochemical Functions of the Membrane-Binding Domain of CARMIL
Article Snippet: .. Liposomes were then passed through a hand-extruder (Genizer, Avanti Research, #610000, Alabaster, AL) and through a 0.2 μm polycarbonate membrane (Avanti Research, #610006, Alabaster, AL) nine times and collected for use. ..

Article Title: CARMIL Membrane-Binding Domain Regulates Capping Protein and Actin Assembly.
Article Snippet: .. Liposomes were then passed through a hand-extruder (Genizer, Avanti Research, #610000, Alabaster, AL) and through a 0.2 μm polycarbonate membrane (Avanti Research, #610006, Alabaster, AL) nine times and collected for use. ..

Pore Size:

Article Title: Structural and biochemical analysis of ligand binding in yeast Niemann-Pick type C1-related protein
Article Snippet: .. Before use, liposomes were extruded by passing 20x through a 100 nm pore size polycarbonate filter (Whatman, Nucleopore) using a hand extruder (Avanti Polar Lipids). ..

Article Title: Yck3 casein kinase-mediated phosphorylation determines Ivy1 localization and function at endosomes and the vacuole.
Article Snippet: The Saccharomyces cerevisiae casein kinase protein Yck3 is a central regulator at the vacuole that phosphorylates several proteins involved in membrane trafficking.. Here, we set out to identify novel substrates of this protein.. We found that endogenously tagged Yck3 localized not only at the vacuole, but also on endosomes.

Article Title: The sterol transporter STARD3 transports sphingosine at ER-lysosome contact sites
Article Snippet: .. Before use, liposomes were extruded by passing 20x through a 100 nm pore size polycarbonate filter (Whatman - Nuclepore) using a hand extruder (Avanti Polar Lipids). ..

Article Title: VAP-A intrinsically disordered regions enable versatile tethering at membrane contact sites.
Article Snippet: .. Liposomes were extruded through 0.1 mm pore size polycarbonate filters using hand extruder (Avanti Polar Lipids) and were used within 1-2 days. .. Full length VAP-A reconstitution into proteoliposomes VAP-AFL WT or sL1/2 were reconstituted by detergent removal using BioBeads as described previously in lipid mixture containing egg phosphatidylcholine (PC) and brain phosphatidylserine (PS) (95/5 mol%) for cryo-EM experiment or egg PC, brain PS, DGS NTA(Ni), cholesterol (93/5/2/10 mol%) for in vitro lipid transfer assay.

Concentration Assay:

Article Title: An ER-Horse Detonating Stress Cascade for Hepatocellular Carcinoma Nanotherapy.
Article Snippet: .. After mixing with siGRP94 (dissolved in 0.01 M PBS; the final concentration was 10 μM), the suspension was kept at 4 °C and extruded five times through a polycarbonate filter of 1.0 μm, five times through a polycarbonate filter of 0.5 μm, and five times through a polycarbonate filter of 0.2 μm using an Avanti Hand Extruder (Avanti Polar Lipids). ..

Article Title: The inner nuclear membrane-associated protein Csa1 regulates lipid droplet biogenesis and nuclear shape in a Sir2-dependent manner
Article Snippet: .. The liposome suspension was then extruded sequentially through 400, 200, 100, and 50 nm polycarbonate filters (Cat#610007, 610006, 610005, and 610003, respectively) using a hand extruder (Avanti, cat610000,) at a final lipid concentration of 10 mg/ml. ..

Suspension:

Article Title: An ER-Horse Detonating Stress Cascade for Hepatocellular Carcinoma Nanotherapy.
Article Snippet: .. After mixing with siGRP94 (dissolved in 0.01 M PBS; the final concentration was 10 μM), the suspension was kept at 4 °C and extruded five times through a polycarbonate filter of 1.0 μm, five times through a polycarbonate filter of 0.5 μm, and five times through a polycarbonate filter of 0.2 μm using an Avanti Hand Extruder (Avanti Polar Lipids). ..

Article Title: The inner nuclear membrane-associated protein Csa1 regulates lipid droplet biogenesis and nuclear shape in a Sir2-dependent manner
Article Snippet: .. The liposome suspension was then extruded sequentially through 400, 200, 100, and 50 nm polycarbonate filters (Cat#610007, 610006, 610005, and 610003, respectively) using a hand extruder (Avanti, cat610000,) at a final lipid concentration of 10 mg/ml. ..



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